https://www.linkedin.com/in/lorendzoguidi/
Contact mail – office
Ciclo
38 (XXXVIII)
Curriculum:
Materials and Chemistry
Title of the PhD Project:
Development of nanoparticles for
precision medicine applications and computational modeling of their distribution and drug release in a physiopathological environment
Supervisor(s)
Elisabetta Rosellini, Ph.D.
Prof. Chiara Galletti
Prof. Maria Grazia Cascone
Abstract of the PhD project: (link video?)
A multitude of sight-threatening retinal diseases, affecting hundreds of millions around the globe, lack effective pharmacological treatments due to ocular barriers and common drug delivery limitations. Polymeric nanoparticles (PNPs) are versatile drug carriers with sustained drug release profiles and tunable physicochemical properties which have been explored for ocular drug delivery to both anterior and posterior ocular tissues. PNPs can incorporate a wide range of drugs and overcome the challenges of conventional retinal drug delivery. Moreover, PNPs can be engineered to respond to specific stimuli such as ultraviolet, visible, or near-infrared light, and allow precise spatiotemporal control of the drug release, enabling tailored treatment regimens and reducing the number of required administrations. The objective of this study is to develop NIR light-triggered drug-loaded polymeric nanoparticles to treat retinal diseases through bulk and microfluidic methods. The PNPs will be fully characterized through SEM imaging, spectral analysis, dynamic light scattering and zeta potential measurements in order to guarantee a thorough understanding of their potential interactions with the human body. The drug release kinetic of the developed PNPs will be mathematically fitted through validated models, and the biodistribution of the PNPs will be computationally simulated through the FLUENT Discrete Phase Modeling tool to predict their behaviour in physiologically relevant geometries and their drug release profiles, thus reducing the need for in vivo testing following the 3Rs principle (replacement, reduction, refinement).
Teaching experience:
Supervision of the Master Thesis work of Gaia Riccio
Publications
Scopus:
https://www.scopus.com/authid/detail.uri?authorId=58683378900
Orcid ID
https://orcid.org/0009-0003-5544-4557
Articles
- Rosellini E., Cascone M.G., Guidi L., Schubert D.W., Roether J.A., Boccaccini A.R.
Mending a broken heart by biomimetic 3D printed natural biomaterial-based cardiac patches: a review
(2023) Frontiers in Bioengineering and Biotechnology, 11, art. no. 1254739
DOI: 10.3389/fbioe.2023.1254739
- Guidi L., Cascone M.G., Rosellini E.
Light-responsive polymeric nanoparticles for retinal drug delivery: design cues, challenges and future perspectives
(2024) Heliyon, 10 (5), art. no. e26616
DOI: 10.1016/j.heliyon.2024.e26616
- Guidi L., Rosellini E., Vozzi G., De Maria C., Fortunato G.M., Lapomarda A., Lazzeri L., Cascone M.G.
3D printing of a nanomaterials-embedded gelatin/pectin hydrogel for the development of an in vitro drug testing platform
(2023) Convegno Nazionale di Bioingegneria
Conferences
- “Nanomateriali Farmaceutici, Biomedici e Cosmetici - Manufacturing & Quality”
13 June 2023; Università degli Studi di Parma; Participation: Online
- “VIII Congress of the National Group of Bioengineering (GNB)”
21-23 June 2023; Padova; Participation: Poster Presentation; Title: “3D bioprinting of a nanomaterials-embedded gelatin/pectin hydrogel for the development of an in vitro drug testing platform”; Authors: Lorenzo Guidi, Elisabetta Rosellini, Giovanni Vozzi, Carmelo De Maria, Gabriele Maria Fortunato, Anna Lapomarda, Luigi Lazzeri, Maria Grazia Cascone
- “Ophthalmic formulations: Challenges and Advances”
14 July 2023; Location: Pharmacy Department (University of Pisa); Participation: on site